We carry a full array of high-quality laboratory analyzers, reagents and kits optimized to fulfill the medical testing needs of your organization.

Our lines of instruments and reagents include: hematology, electrolyte , RT-PCR analyzers , Flow cytometry antibodies , Research Elisa kits and antibodies.

Hematology analyzers

Electrolyte analyzers

RT-PCR analyzers

Flow cytometry antibodies

Research antibodies and kits(IHC-P,WB,FACS,IF/ICC and Elisa kits)

Immunohistochemistry begins with collection of a sample of tissue from the patient. The integrity of the sample tissue is paramount to the accuracy of the procedure. Fresh tissue samples are hence typically examined as soon as possible. However, frozen or preserved tissue samples may be used in some cases.

The sample is sliced thinly to achieve a one cell thickness and placed on a glass slide. The sample is then introduced to antibodies, which bind to antigens in the cell. A microscope is used to locate these bound antibodies, which are visible either through fluorescence or chemical reactions.

Based on the binding patterns present in the immunohistochemistry tissue sample, pathologists can glean an enormous amount of information. For example, the process allows experts to determine the origin of the abnormal cells, which often leads to a successful diagnosis.

Two immunohistochemistry methods are employed direct and indirect. The direct IHC method is a straightforward one-step process that creates a direct reaction between the antigen and the labeled antibody. This method is nicely simple but is less sensitive than the more complex indirect method. For this reason, the direct method is rarely used today.

The indirect method requires the use of two antibodies a primary unlabeled antibody and a secondary labeled antibody. The addition of the primary antibody allows for signal amplification of the process, which in turn delivers improved sensitivity and more accurate results.

Immunohistochemistry has been a successful technique for marking antigens since the early 1940s. In comparison to similar investigative cell techniques, IHC provides improved precision in locating specific proteins of interest associated with the identification of cancerous tumors. For example, studies have shown that IHC is more sensitive in the diagnosis of breast cancer when compared to alternative tests such as biochemical determination and dextran-coated charcoal assay (DCC).

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